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Barack Obama has said a trade deal between the UK and US would be at the "back of the queue" if the UK left the European Union.
The President said a free trade arrangement would not happen "any time soon" in the event of Brexit.
Speaking alongside Prime Minister David Cameron at the Foreign Office, he said: "I think it's fair to say that maybe some point down the line there might be a UK-US trade agreement.
"But it's not going to happen any time soon because our focus is in negotiating with a big bloc, the European Union, to get a trade agreement done.
"The UK is going to be in the back of the queue."
Trying to do "piecemeal trade agreements" was "hugely inefficient", the President said.
He added, however, that the referendum was a decision for the British people and his comments were "not a threat" or an attempt to "fix any votes".
He was speaking after his comments in the Daily Telegraph urging the UK to stay in the Union triggered a furious row.
The remarks enraged many Tory eurosceptics, but delighted pro-EU campaigners.
President Obama again made no bones about his views as he faced reporters at the Foreign Office.
He said the matter was of of "deep interest" to the US and that the UK was at its best when helping lead Europe.
Read more at http://news.sky.com/story/1683118/obama-uk-us-trade-deal-would-be-back-of-queue